Nicking reaction catalysed by the MobA89K and MobC89K proteins. The cleaved strand is then unwound because of the helicase and sent to the 89K-encoded GI-type T4SS machinery. The VirB1-89K part is thought to work as a lytic transglycosylase, domestically opening the peptidoglycan with contributions from A few other element(s) in https://89keinfo62695.uzblog.net/everything-about-89k-44073048