Tobacco rattle virus (TRV) has a bipartite. positive-sense single-stranded RNA genome and is widely used for virus-induced gene silencing (VIGS) in plants. RNA1 of TRV that lacks the gene for the cysteine-rich 16K silencing-suppression protein infects plants systemically in the absence of RNA2. Here. https://www.chiggate.com/elliot-pecan-tree-sale/